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library name : انستیتو تغذیه دانشگاه علوم پزشکی شهید بهشتی
Material Type : Latin Articles
Language of Document : English
Record Number : 61534
Doc. No : 185A
Main Entry : MALEKI RAVASAN N
Title & Author : BLOOD MEAL IDENTIFICATION IN FIELD-CAPTURED SAND FLIES: COMPARISON OF PCR-RFLP AND ELISA ASSAYS [Article]; RASI Y.,SADRAEI J.,MOHTARAMI F
Title : IRANIAN JOURNAL OF ARTHROPOD-BORNE DISEASES
Volume Number : , Vol.3 ؛ No.1
Date : , (2009)
page : : 8 - 18
Abstract : Background: We aimed to develop a PCR-RFLP assay based on available sequences of putative vertebrate hosts to identify blood meals ingested by field female sand fly in the northwest of Iran. In addition, the utility of PCR-RFLP was compared with ELISA as a standard method.Methods: This experimental study was performed in the Insect Molecular Biology Laboratory of School of Public Health, Tehran University of Medical Sciences, Iran in 2006-2007. For PCR-RFLP a set of conserved vertebrate primers were used to amplify a part of the host mitochondrial cytochrome b )cyt b( gene followed by digestion of the PCR products by Hae III enzyme.Results: The PCR-RFLP and ELISA assays revealed that 34percent and 27percent of field-collected sand flies had fed on humans, respectively. Additionally, PCR-RFLP assays could reveal specific host DNA as well as the components of mixed blood meals. Results of PCR-RFLP assay showed that the sand flies had fed on cow )54percent(, human )10percent(, dog )4percent(, human and cow )21percent(, dog and cow )14percent(, and human and dog )3percent(. Conclusion: The results can provide a novel method for rapid diagnosis of blood meal taken by sandflies. The advantages and limitations of PCR and ELISA assays are discussed.
Descriptor : LEISHMANIASIS
Descriptor : SAND FLIES
Descriptor : BLOOD MEAL
Descriptor : PCR
Descriptor : ELISA
Descriptor : IRAN
Added Entry : OSHAGHI M.A
: JAVADIAN E
 
 
 
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