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library name : انستیتو تغذیه دانشگاه علوم پزشکی شهید بهشتی
Material Type : Latin Articles
Language of Document : English
Record Number : 61647
Doc. No : 298A
Main Entry : IMANI FOULADI A.A
Title & Author : DETECTION OF ENTEROTOXIGENIC STAPHYLOCOCCUS AUREUS ISOLATES IN DOMESTIC DAIRY PRODUCTS [Article]
Title : IRANIAN JOURNAL OF MICROBIOLOGY
Volume Number : , Vol.2 ؛ No.3
Date : , (2010)
page : : 135 - 140
Abstract : Background and objectives: Staphylococcus aureusis a one of THE most frequent causes of food poisoning )FP( in dairy products. The main etiologic agents of FP are staphylococcal enterotoxins )SE(. There are different types of SE; types A )SEA( and B )SEB( are the most clinically important enterotoxins. Traditional dairy products are still produced in small batches and sold by some vendors without a permit from the Ministry of Health. This study focuses on the molecular and serological detection of enterotoxigenic Staphylococcus aureus SEA and SEB genes and its products, respectively from samples of such traditional products. Materials and Methods: 100 samples from dairy products were produced under sterile conditions via traditional methods and were transported to the laboratory. The samples were cultured and identified by routine bacteriological methods. The isolated bacteria were evaluated by PCR tests for detection of the genes encoding SEA and SEB. Subsequently, the ability of these strains to produce enterotoxin was examined by Sacعs culture method and was confirmed by Sigel Radial Immounodiffussion )SRID(. Results: The results indicated that 32percent of the dairy products were contaminated by S. aureus )cream 18percent , cheese 10percent, milk 4percent(. The PCR results showed that 15.6percent of the S. aureus isolates possessed the SEA gene, 9.3percent had the SEB gene, and 6.2percent possessed both genes. The evaluation of enterotoxin production indicated that 80percent of SEA and 33percent of SEB genes were expressed.Conclusion: Enterotoxins SEA and SEB are heat stable and consequently; heating has no effect on dairy products contaminated by entertoxins. Subsequently, gastritis may occur within several hours after consumption. Our findings suggest that PCR is a rapid, sensitive, specific, and inexpensive method for detecting SE and can replace the traditional assays.
Descriptor : STAPHYLOCOCCUS AUREUS
Descriptor : ENTEROTOXIN
Descriptor : FOOD POISONING
Descriptor : DAIRY PRODUCTS
Added Entry : TAVAKOLI H.R
: NADERI A
 
 
 
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