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library name : انستیتو تغذیه دانشگاه علوم پزشکی شهید بهشتی
Material Type : Latin Articles
Language of Document : English
Record Number : 61657
Doc. No : 308A
Main Entry : BAHMANZADEGAN JAHROMI A
Title & Author : DETERMINATION OF HYOSCYAMINE AND SCOPOLAMINE IN FOUR HYOSCYAMUS SPECIES FROM IRAN [Article]
Title : IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)
Volume Number : , Vol.8 ؛ No.1
Date : , (2009)
page : : 65 - 70
Abstract : The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25percent ammonium hydroxide 15:5:1 )V/V/V(. HPLC separation was performed on Eurospher C18 reversed phase column. An isocratic mixture of triethylammonium phosphate buffer )30 Mm, pH 6.2( and acetonitrile )75:25(, was used as the eluent. Hyoscyamine and scopolamine were determined by the external standard method at 210 nm. All the four mentioned Hyoscyamus species contained hyoscyamine and scopolamine, but in different amounts. Scopolamine was the predominant tropane alkaloid in H. pusillus, H. niger and H. kurdicus, while, H. reticulatus contained a higher amount of hyoscyamine.
Descriptor : HYOSCYAMUS
Descriptor : TROPANE ALKALOID
Descriptor : HYOSCYAMINE
Descriptor : SCOPOLAMINE
Descriptor : HPLC
Added Entry : SEFIDKON F
: SONBOLI A.
 
 
 
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