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library name : انستیتو تغذیه دانشگاه علوم پزشکی شهید بهشتی
Material Type : Latin Articles
Language of Document : English
Record Number : 61718
Doc. No : 370A
Main Entry : EYN ELAHI N
Title & Author : EFFECT OF MERCURIC CHLORIDE ON KINETIC PROPERTIES OF HORSERADISH PEROXIDASE [Article]
Title : IRANIAN JOURNAL OF PUBLIC HEALTH
Volume Number : , Vol.35 ؛ No.2
Date : , (2006)
page : : 49 - 56
Abstract : Mercury is one of the three major environmental metal poisons, and mercuric chloride is a highly reactive compound which can harm cells by a variety of mechanisms including direct interaction with sulphydryl groups of proteins and enzymes, therefore affecting the enzymatic activity. This study focused on the effect of Hg++ on horseradish peroxidase )donor: hydrogen peroxide oxidoreductase, EC 1.11.1.7( )HRP( )Isoenzyme C( activity. In the presence of 88 mM hydrogen peroxide Km for o-dianisidine oxidation was 0.05 millimolar and Vmax was 8.5 ?M.s-1. Incubation of the enzyme with 1 to 100 millimolar mercuric chloride for 5-20- and 60 min resulted in progressive inhibition of the enzymatic activity. At low Hg++ concentrations the inhibition was reversible by excess substrate, while at high Hg++ concentration the inhibition was not reversible. Results also indicated that the type of inhibition depended on the duration of incubation of the enzyme with metal ion and on the Hg++ concentration. So we could conclude that the type of inhibition changed from noncompetitive to mix with increased incubation time and increased metal concentration.
Descriptor : HORSERADISH PEROXIDASE
Descriptor : MERCURIC CHLORIDE
Descriptor : ENZYME INHIBITION
Descriptor : O DIANISIDINE
Added Entry : ABBASI S
: VAEZ ZADEH F
 
 
 
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