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library name : انستیتو تغذیه دانشگاه علوم پزشکی شهید بهشتی
Material Type : Latin Articles
Language of Document : English
Record Number : 61967
Doc. No : 623A
Main Entry : RAZAFSHA M.,
Title & Author : INHIBITORY EFFECTS OF AQUEOUS ONION AND GARLIC EXTRACTS ON GROWTH AND KERATINASE ACTIVITY IN TRICHOPHYTON MENTAGROPHYTES [Article]
Title : IRANIAN BIOMEDICAL JOURNAL
Volume Number : , Vol.7 ؛ No.3
Date : , (2003)
page : : 113 - 118
Abstract : The effect of onion and garlic extracts on fungal growth and keratinolytic activity wasع‌ژ studied in Trichophyton mentagrophytes as one of the major etiologic agents ofع‌ژ human and animal dermatophytosis in Iran and other parts of the world. In order toع‌ژ find out the best keratinase producer for further steps, culture conditions for 30 strainsع‌ژ of T. mentagrophytes isolated from human dermatophytosis were optimized onع‌ژ specific solid and liquid media. All of the isolates produced the enzyme on bothع‌ژ selective culture media. The maximum keratinolytic activity at submerged cultivationع‌ژ was reported for cultures of T. mentagrophytes isolate No. 1 grown for a 12-dayع‌ژ period at 32░آC. Extracellular keratinase activity was in the range of 0.28 to 2.18 u/mgع‌ژ protein in different isolates at predetermined optimal conditions. The growth of T.ع‌ژ mentagrophytes isolate No. 1 was inhibited in the presence of various concentrationsع‌ژ of onion and garlic extracts. This inhibition reached to a maximum of 100percent for bothع‌ژ extracts at 10percent v/v concentrations. Keratinase synthesis was also inhibited by twoع‌ژ extracts as a dose-dependent manner with maximums about 58.54 and 71.36 percentع‌ژ at 5percent concentrations, accordingly. In contrast to the fungal growth, keratinolyticع‌ژ activity was inhibited more by garlic as compared with onion extract. This is the firstع‌ژ report on keratinase inhibition by these two natural compounds. Since fungal growthع‌ژ and keratinolytic activity are important factors in pathogenesis of the dermatophytes,ع‌ژ their inhibition by onion and garlic indicate that these substances may have potentialع‌ژ values for treatment of human and animal dermatophytosis.ع‌ع
Descriptor : TRICHOPHYTON MENTAGROPHYTES
Descriptor : KERATINASE
Descriptor : ONION
Descriptor : GARLIC
Descriptor : DERMATOPHYTOSIS
Added Entry : SHAMS GHAHFAROKHI M
 
 
 
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